Praeruptorin D (PD), a major pyranocoumarin isolated from Radix Peucedani,\r\nexhibited antitumor and anti-inflammatory activities. The aim of this study was to\r\ninvestigate the pharmacokinetics and tissue distribution of PD in rats following intravenous\r\n(i.v.) administration. The levels of PD in plasma and tissues were measured by a simple\r\nand sensitive reversed-phase high-performance liquid chromatography (HPLC) method.\r\nThe biosamples were treated by liquid-liquid extraction (LLE) with methyl tert-butyl ether\r\n(MTBE) and osthole was used as the internal standard (IS). The chromatographic\r\nseparation was accomplished on a reversed-phase C18 column using methanol-water\r\n(75:25, v/v) as mobile phase at a flow rate of 0.8 mL/min and ultraviolet detection wave\r\nlength was set at 323 nm. The results demonstrate that this method has excellent specificity,\r\nlinearity, precision, accuracy and recovery. The pharmacokinetic study found that PD fitted\r\nwell into a two-compartment model with a fast distribution phase and a relative slow\r\nelimination phase. Tissue distribution showed that the highest concentration was observed\r\nin the lung, followed by heart, liver and kidney. Furthermore, PD can also be detected in the\r\nbrain, which indicated that PD could cross the blood-brain barrier after i.v. administration.
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